ChinaPeptides
Polyclonal Antibody laboratory

Polyclonal Antibody

Polyclonal antibodies raised against peptide or protein antigens.

Polyclonal antibody services are suitable when a broader epitope response is useful for research assays. Peptide antigens are commonly conjugated to KLH before immunization.

Antiserum can be affinity-purified against the immunizing peptide when requested. That step reduces carrier-reactive antibodies and is often worth specifying for blot work.

A polyclonal lot is finite. If you later need a renewable reagent, plan a monoclonal follow-up rather than assuming a second bleed will match the first.

Host choice, bleed schedule and whether a pre-immune sample is retained are part of the written scope. We do not invent a single “standard titer” that applies to every peptide.

For peptide programs, the screening peptide should be the free peptide or a second carrier, not only the KLH conjugate used for immunization. That distinction belongs on the antibody production page and in the quote.

When polyclonal is enough

Use a polyclonal reagent when you need a first research antibody quickly, when the epitope may be conformationally mixed, or when the experiment is a one-time blot or ELISA development. Do not use it when you already know you will need the same clone for years.

Affinity purification

Antigen-affinity purification is requested when carrier-reactive antibodies would confuse the assay. It consumes peptide and should be planned at synthesis so that enough antigen remains for the column.

Frequently asked questions

How much peptide is needed for immunization and purification?

That depends on host number and whether affinity purification is requested. State both in the inquiry.

Can I order a second identical lot later?

A later bleed is related, not identical. Treat it as a new lot and re-qualify the assay.

Is this for human use?

No. Materials are research reagents only.

Need a Custom Antibody Program?

Share the antigen, host and intended research application.